DGaO·Proceedings ISSN 1614-8436
DE
Poster P52

Investigations on Label-Free Identification of Subcellular Tumor Cell Structures by Digital Holographic Phase Contrast Microscopy

Sabine Przibilla1, Bjoern Kemper1, Christina E. Rommel2, Steffi Ketelhut1, Angelika Vollmer1, Patrik Langehanenberg1, Jürgen Schnekenburger2, Gert von Bally1
  1. 1Center for Biomedical Optics and Photonics, University of Münster
  2. 2Department of Medicine B, University of Münster

bkemper [at] uni-muenster [dot] de

Digital holographic microscopy (DHM) offers a quantitative phase contrast for minimally invasive analysis of living single cells and cell cultures with low demands on (label-free) sample preparation. In order to interpret DHM images for analysis of subcellular structures, comparative investigations were performed using fluorescence microscopy with DAPI-staining (4',6-Diamidino-2-phenylindol), since the latter allows identification of cell components containing DNA. Results from statistical studies on adherent pancreas tumor cells with fluorescence microscopy and DHM show that nucleus components can be identified label-free in digital holographic phase contrast images.

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@inproceedings{przibilla2009p52,
  author     = {Przibilla, Sabine and Kemper, Bjoern and Rommel, Christina E. and Ketelhut, Steffi and Vollmer, Angelika and Langehanenberg, Patrik and Schnekenburger, Jürgen and von Bally, Gert},
  title      = {{Investigations on Label-Free Identification of Subcellular Tumor Cell Structures by Digital Holographic Phase Contrast Microscopy}},
  booktitle  = {DGaO-Proceedings 2009},
  series     = {DGaO-Proceedings},
  eventtitle = {110. Jahrestagung der DGaO},
  eventdate  = {2009-06-02/2009-06-05},
  venue      = {Brescia},
  publisher  = {Deutsche Gesellschaft für angewandte Optik e.V.},
  year       = {2009},
  pages      = {P52},
  url        = {https://dgao-proceedings.de/paper/110-p52}
}
110. Annual Conference of the DGaO · Brescia · 2009